lenticrisprv2 (Addgene inc)
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lenticrisprv2
Lenticrisprv2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 6093 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lenticrispr+v2/lentiCRISPR+v2+(Plasmid+%2352961)/pmc12990376-93-0-2
Average 96 stars, based on 6093 article reviews
Lenticrisprv2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 6093 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lenticrispr+v2/lentiCRISPR+v2+(Plasmid+%2352961)/pmc12990376-93-0-2
Average 96 stars, based on 6093 article reviews
lenticrisprv2 - by Bioz Stars,
2026-09
96/100 stars
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Transfection:Article Title: Betrixaban activates cGAS and ERVs to promote dual nucleic-sensing antiviral immunity. Article Snippet: 14 EMBO Molecular Medicine © The Author(s) The oligos were annealed and cloned into the lentiCRISPR v2 vector, which had been digested with the BsmBI enzyme (NEB). .. For lentivirus production, 293T cells were transfected with the following plasmids: Plasmid Preparation:Article Title: Betrixaban activates cGAS and ERVs to promote dual nucleic-sensing antiviral immunity. Article Snippet: 14 EMBO Molecular Medicine © The Author(s) The oligos were annealed and cloned into the lentiCRISPR v2 vector, which had been digested with the BsmBI enzyme (NEB). .. For lentivirus production, 293T cells were transfected with the following plasmids: Article Title: The Protein Tyrosine Phosphatase CD45 promotes PMN Transepithelial Migration, Antimicrobial Function and Colonic Mucosal Repair Article Snippet: .. The SpCas9 of Article Title: BCR::ABL1 tyrosine kinase inhibitors induce ribosome collisions to activate ZAK-dependent ribotoxic stress and apoptosis in chronic myeloid leukemia. Article Snippet: P210 pcDNA3 (Addgene plasmid #27481) was a gift from Warren Pear [55]. pLentiPGK Puro DEST p38KTRClover (Addgene plasmid #59152) was a gift from Markus Covert [56]. .. The oligonucleotide pair encoding a ZAK-specific sgRNA (5′-ATGGATATCACAGGACAAGG-3′) was synthesized (Macrogen) and cloned into Knock-Out:Article Title: Tumor-intrinsic metabolic pathways essential for tumorigenesis and resistance to anti-PD1 in oncogenic Kras-driven lung adenocarcinoma. Article Snippet: .. Generation of knock-out cell lines sgRNAs targeting Pla2g4a, as well as a non-targeting (NT) control sgRNA, were cloned into Control:Article Title: Tumor-intrinsic metabolic pathways essential for tumorigenesis and resistance to anti-PD1 in oncogenic Kras-driven lung adenocarcinoma. Article Snippet: .. Generation of knock-out cell lines sgRNAs targeting Pla2g4a, as well as a non-targeting (NT) control sgRNA, were cloned into Clone Assay:Article Title: Tumor-intrinsic metabolic pathways essential for tumorigenesis and resistance to anti-PD1 in oncogenic Kras-driven lung adenocarcinoma. Article Snippet: .. Generation of knock-out cell lines sgRNAs targeting Pla2g4a, as well as a non-targeting (NT) control sgRNA, were cloned into Article Title: BCR::ABL1 tyrosine kinase inhibitors induce ribosome collisions to activate ZAK-dependent ribotoxic stress and apoptosis in chronic myeloid leukemia. Article Snippet: P210 pcDNA3 (Addgene plasmid #27481) was a gift from Warren Pear [55]. pLentiPGK Puro DEST p38KTRClover (Addgene plasmid #59152) was a gift from Markus Covert [56]. .. The oligonucleotide pair encoding a ZAK-specific sgRNA (5′-ATGGATATCACAGGACAAGG-3′) was synthesized (Macrogen) and cloned into Article Title: TRIM21 is a molecular rheostat for influenza A virus replication Article Snippet: Influenza viruses were propagated in Madin Darby Canine Kidney (MDCK) cells in DMEM supplemented with 1 μg/mL Tosyl Phenylalanyl Chloromethyl Ketone (TPCK)-treated trypsin (Thermo Scientific). .. These were cloned into Polymerase Chain Reaction:Article Title: Tumor-intrinsic metabolic pathways essential for tumorigenesis and resistance to anti-PD1 in oncogenic Kras-driven lung adenocarcinoma. Article Snippet: .. Generation of knock-out cell lines sgRNAs targeting Pla2g4a, as well as a non-targeting (NT) control sgRNA, were cloned into Modification:Article Title: The Protein Tyrosine Phosphatase CD45 promotes PMN Transepithelial Migration, Antimicrobial Function and Colonic Mucosal Repair Article Snippet: .. The SpCas9 of Synthesized:Article Title: BCR::ABL1 tyrosine kinase inhibitors induce ribosome collisions to activate ZAK-dependent ribotoxic stress and apoptosis in chronic myeloid leukemia. Article Snippet: P210 pcDNA3 (Addgene plasmid #27481) was a gift from Warren Pear [55]. pLentiPGK Puro DEST p38KTRClover (Addgene plasmid #59152) was a gift from Markus Covert [56]. .. The oligonucleotide pair encoding a ZAK-specific sgRNA (5′-ATGGATATCACAGGACAAGG-3′) was synthesized (Macrogen) and cloned into Recombinant:Article Title: BCR::ABL1 tyrosine kinase inhibitors induce ribosome collisions to activate ZAK-dependent ribotoxic stress and apoptosis in chronic myeloid leukemia. Article Snippet: P210 pcDNA3 (Addgene plasmid #27481) was a gift from Warren Pear [55]. pLentiPGK Puro DEST p38KTRClover (Addgene plasmid #59152) was a gift from Markus Covert [56]. .. The oligonucleotide pair encoding a ZAK-specific sgRNA (5′-ATGGATATCACAGGACAAGG-3′) was synthesized (Macrogen) and cloned into other:Article Title: A single-cell CRISPR screen defines a gene regulatory network governing human pluripotency in primed and naive cells. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Cell lines hpES10 Amit et al.63 N/A HEK293T Laboratory of Robert Weinberg RRID: CVCL_0063 primed Cas9-EGFP-hPSCs This study N/A primed ZIM3-dCas9-mCherry-hPSCs Sherman et al.64 N/A Oligonucleotides sgRNA targeting ZNF281 (CRISPRi protospacer) hCRISPRi-v2 5′-GTATTAACTGGAGGCGACGG-3′ sgRNA targeting ETV4 (CRISPRi protospacer) hCRISPRi-v2 5′-GCCTCAGGTGAGGCTGCGGG-3′ sgRNA targeting Gal4 promoter (CRISPRi negative control protospacer) hCRISPRi-v2 5′-GAACGACTAGTTAGGCGTGT-3′ sgRNA targeting SMARCC1 (CRISPR KO; perturb-seq gRNA_10) This study See Table S2 sgRNA targeting TGIF1 (CRISPR KO; perturb-seq gRNA_3) This study See Table S2 sgRNA targeting ZNF281 (CRISPR KO; perturb-seq gRNA_7) This study See Table S2 Perturb-seq gRNA library composition This study See Table S2 qPCR primers: POU5F1_F This study 5′-GTGGAGGAAGCTGACAACAA-3′ qPCR primers: POU5F1_R This study 5′-TCTCCAGGTTGCCTCTCACT-3′ qPCR primers: NANOG_F This study 5′-CATGAGTGTGGATCCAGCTTG-3′ qPCR primers: NANOG_R This study 5′-CCTGAATAAGCAGATCCATGG-3′ qPCR primers: PRDM14_F This study 5′-ACCGGCCTCACAAGTGTTCT-3′ qPCR primers: PRDM14_R This study 5′-GAGTGCTGCCTGATGTGTGT-3′ qPCR primers: ETV4_F This study 5′-CACCTTCAGCAGCAAATCGC-3′ qPCR primers: ETV4_R This study 5′-ATCTTCAGAGTCGAGGGGCG-3′ qPCR primers: ZNF281_F This study 5′-CGCAATGCGTGTTATCCTCC-3′ qPCR primers: ZNF281_R This study 5′-CCGCGGGTTTCTCCTGTTTG-3′ qPCR primers: GAPDH_F This study 5′-AGCCACATCGCTCAGACACC-3′ qPCR primers: GAPDH_R This study 5′-GTACTCAGCGCCAGCATCG-3′ Recombinant DNA lentiCas9-EGFP A gift from Article Title: Microhomology-mediated end joining acts directly on replication forks to repair single-ended double-strand breaks. Article Snippet: In brief Li et al. uncover a new mechanism, forkMMEJ, at broken replication forks that repairs seDSBs with characteristics distinct from the canonical MMEJ (cMMEJ).. This study provides insights into replication-associated DSB repair, implicates microhomology-mediated chromosomal rearrangements in cancer, and offers potential therapeutic strategies to selectively target cancer cells.. ATR Suppress Broken forks |